Isolation/Culture of Virus
How is the virus isolated or cultured in a lab?
Dan Szyprowski writes: One aliquot of each of the brain and spleen tissue samples collected from the diseased emu was cultured for aerobic and anaerobic bacteria. The other aliquot was weighed and homogenized in PBS. The isolation for the virus was shown by the brain and spleen tissue samples given an allantoic sac of embryonated SPF leghorn chicken eggs.
Why is it more challenging to culture this particular strain termed bird flu?
Dan Szyprowski writes: It is very hard to culture this type of flu because it is still very complicated and not a lot of people know about this certain disease.